Rafa Marquez scored on a header in the 89th minute, and Mexico beat the United States 2-1 Friday night in the Americans' first home loss in World Cup qualifying since 2001.
Miguel Layun put Mexico ahead in the 20th minute, but Bobby Wood tied the score in the 49th.
The U.S. dominated the second half before the 37-year-old Marquez, unmarked and drifting across the penalty area, got the back of his head on Layun's corner kick and lifted the ball over goalkeeper Brad Guzan.
Guzan lost the goalkeeper job to Tim Howard, who started at the last two World Cups. But Howard injured his right leg on a goal kick and was replaced in the 40th minute.
The U.S. had beaten Mexico four straight times by 2-0 scores in home qualifiers — all at Columbus — and the Americans had been 30-0-2 at home in qualifying since a 3-2 loss to Honduras at Washington's RFK Stadium in September 2001.